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Modulation of collagen production by fibroblasts. Effects of chronic exposure to agonists that increase intracellular cyclic AMP.
| Publication Type | Academic Article |
| Authors | Saltzman L, Moss J, Berg R, Hom B, Crystal R |
| Journal | Biochem J |
| Volume | 204 |
| Issue | 1 |
| Pagination | 25-30 |
| Date Published | 04/15/1982 |
| ISSN | 0264-6021 |
| Keywords | Collagen, Cyclic AMP, Fibroblasts, Isoproterenol, Prostaglandins E |
| Abstract | Cultured human lung fibroblasts were evaluated for their responsiveness to isoprenaline (isoproterenol) or prostaglandin E2 before and after chronic incubation with the agonist. Cells incubated for 6 h with either agonist were suppressed in terms of collagen production and exhibited increased intracellular cyclic AMP. Cells incubated for 72 h with the agonist and then re-challenged for 6 h with the same agonist did not demonstrate suppressed collagen production or increased cyclic AMP. Cells incubated for 72 h with isoprenaline still responded to prostaglandin E2 when challenged for 6 h; however, when the order of agonist exposure was reversed, cells incubated with prostaglandin E2 did not respond to a challenge by isoprenaline. If cells were allowed to recover for 48 h without the agonist after a 72 h chronic incubation, they recovered their responsiveness to the agonist. The results indicate that, although cultured fibroblasts may become desensitized to one agonist, they may retain their sensitivity to a second agonist and chronic suppression of collagen production may be achieved by alternate exposure to isoprenaline and prostaglandin E2. |
| DOI | 10.1042/bj2040025 |
| PubMed ID | 6288014 |
| PubMed Central ID | PMC1158311 |