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Procollagen messenger RNA levels and activity and collagen synthesis during the fetal development of sheep lung, tendon, and skin.
| Publication Type | Academic Article |
| Authors | Tolstoshev P, Haber R, Trapnell B, Crystal R |
| Journal | J Biol Chem |
| Volume | 256 |
| Issue | 18 |
| Pagination | 9672-9 |
| Date Published | 09/25/1981 |
| ISSN | 0021-9258 |
| Keywords | Collagen, Lung, Procollagen, RNA, Messenger, Skin, Tendons |
| Abstract | The rates of type I collagen synthesis in sheep lung, tendon, and skin were evaluated during the latter half of fetal development and compared with the levels of type I procollagen mRNA, quantified by molecular hybridization with a type I procollagen specific complementary DNA, and with the activity of total procollagen mRNA measured by in vitro cell-free translation. In the lung and tendon, the levels of type I procollagen mRNA and activity of total procollagen mRNA parallel collagen synthesis during development. In the skin, however, type I collagen synthesis declines sharply during fetal development, but both type I procollagen mRNA levels and total procollagen mRNA activity remain at the high levels of early development. These observations suggest that in developing lung and tendon, type I procollagen mRNA levels are likely the major determinants of the levels of type I collagen synthesis. In contrast, the dichotomy between type I procollagen mRNA levels and rates of type I collagen synthesis in the developing sheep skin suggest the skin utilizes mechanisms in addition to mRNA levels to modulate expression of the type I collagen gene. |
| PubMed ID | 7287703 |