About Us
The Department of Genetic Medicine at Weill Cornell leads a dynamic and innovative translational research program, advancing diverse fields such as Genetic Therapy and Personalized Medicine.
Down-regulation of cystic fibrosis gene mRNA transcript levels and induction of the cystic fibrosis chloride secretory phenotype in epithelial cells by phorbol ester.
| Publication Type | Academic Article |
| Authors | Trapnell B, Zeitlin P, Chu C, Yoshimura K, Nakamura H, Guggino W, Bargon J, Banks T, Dalemans W, Pavirani A |
| Journal | J Biol Chem |
| Volume | 266 |
| Issue | 16 |
| Pagination | 10319-23 |
| Date Published | 06/05/1991 |
| ISSN | 0021-9258 |
| Keywords | Chlorides, Cystic Fibrosis, Down-Regulation, RNA, Messenger, Tetradecanoylphorbol Acetate, Transcription, Genetic |
| Abstract | To evaluate the hypothesis that phorbol myristate acetate (PMA) might modulate the expression of the cystic fibrosis (CF) gene in epithelial cells, we examined the effect of PMA on CF mRNA levels and regulation of Cl- secretion. Strikingly, PMA down-regulated CF mRNA transcript numbers in a dose- and time-dependent manner. Importantly, in parallel with the reduction of CF mRNA levels, PMA-treated cells were unable to up-regulate Cl- secretion in a normal fashion in response to forskolin, an effect which was also dose- and time-dependent. Thus, PMA is capable of modulating expression of the CF gene and induces T84 cells to adopt the "CF phenotype" in regard to regulation of Cl- ion transport. |
| PubMed ID | 2037584 |