Administration of an adenovirus containing the human CFTR cDNA to the respiratory tract of individuals with cystic fibrosis.

Publication Type Academic Article
Authors Crystal R, McElvaney N, Rosenfeld M, Chu C, Mastrangeli A, Hay J, Brody S, Jaffe H, Eissa N, Danel C
Journal Nat Genet
Volume 8
Issue 1
Pagination 42-51
Date Published 09/01/1994
ISSN 1061-4036
Keywords Adenoviridae, Cystic Fibrosis, DNA, Recombinant, Genetic Therapy, Membrane Proteins, Respiratory System
Abstract We have administered a recombinant adenovirus vector (AdCFTR) containing the normal human CFTR cDNA to the nasal and bronchial epithelium of four individuals with cystic fibrosis (CF). We show that this vector can express the CFTR cDNA in the CF respiratory epithelium in vivo. With doses up to 2 x 10(9) pfu, there was no recombination/complementation or shedding of the vector or rise of neutralizing antibody titres. At 2 x 10(9) pfu, a transient systemic and pulmonary syndrome was observed, possibly mediated by interleukin-6. Follow-up at 6-12 months demonstrated no long term adverse effects. Thus, it is feasible to use an adenovirus vector to transfer and express the CFTR cDNA in the respiratory epithelium of individuals with CF. Correction of the CF phenotype of the airway epithelium might be achieved with this strategy.
DOI 10.1038/ng0994-42
PubMed ID 7527271
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