Augmentation of blood platelet levels by intratracheal administration of an adenovirus vector encoding human thrombopoietin cDNA.

Publication Type Academic Article
Authors Cannizzo S, Frey B, Raffi S, Moore M, Eaton D, Suzuki M, Singh R, Mack C, Crystal R
Journal Nat Biotechnol
Volume 15
Issue 6
Pagination 570-3
Date Published 06/01/1997
ISSN 1087-0156
Keywords Gene Transfer Techniques, Platelet Count, Thrombopoietin
Abstract This study was designed to evaluate the hypothesis that administration of a replication-deficient, recombinant adenovirus vector to the epithelial surface of the respiratory tract can be used to deliver a recombinant protein to the systemic circulation in sufficient quantities to evoke a systemic response appropriate to the recombinant protein. We administered AdCMV.TPO-an adenovirus vector containing an expression cassette coding for the human thrombopoietin (TPO) cDNA-to the respiratory epithelium of immunocompetent Balb/c mice. Over the following week, serum human TPO levels were elevated, platelet levels increased more than sixfold, and megakaryocytosis was evident in bone marrow. This strategy may be a useful approach to the nonparenteral administration of a variety of therapeutic recombinant proteins, such as those relevant to clotting, endocrine function, and bone-marrow function.
DOI 10.1038/nbt0697-570
PubMed ID 9181581
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