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Augmentation of blood platelet levels by intratracheal administration of an adenovirus vector encoding human thrombopoietin cDNA.
| Publication Type | Academic Article |
| Authors | Cannizzo S, Frey B, Raffi S, Moore M, Eaton D, Suzuki M, Singh R, Mack C, Crystal R |
| Journal | Nat Biotechnol |
| Volume | 15 |
| Issue | 6 |
| Pagination | 570-3 |
| Date Published | 06/01/1997 |
| ISSN | 1087-0156 |
| Keywords | Gene Transfer Techniques, Platelet Count, Thrombopoietin |
| Abstract | This study was designed to evaluate the hypothesis that administration of a replication-deficient, recombinant adenovirus vector to the epithelial surface of the respiratory tract can be used to deliver a recombinant protein to the systemic circulation in sufficient quantities to evoke a systemic response appropriate to the recombinant protein. We administered AdCMV.TPO-an adenovirus vector containing an expression cassette coding for the human thrombopoietin (TPO) cDNA-to the respiratory epithelium of immunocompetent Balb/c mice. Over the following week, serum human TPO levels were elevated, platelet levels increased more than sixfold, and megakaryocytosis was evident in bone marrow. This strategy may be a useful approach to the nonparenteral administration of a variety of therapeutic recombinant proteins, such as those relevant to clotting, endocrine function, and bone-marrow function. |
| DOI | 10.1038/nbt0697-570 |
| PubMed ID | 9181581 |