About Us
The Department of Genetic Medicine at Weill Cornell leads a dynamic and innovative translational research program, advancing diverse fields such as Genetic Therapy and Personalized Medicine.
A common mechanism for cytoplasmic dynein-dependent microtubule binding shared among adeno-associated virus and adenovirus serotypes.
| Publication Type | Academic Article |
| Authors | Kelkar S, De B, Gao G, Wilson J, Crystal R, Leopold P |
| Journal | J Virol |
| Volume | 80 |
| Issue | 15 |
| Pagination | 7781-5 |
| Date Published | 08/01/2006 |
| ISSN | 0022-538X |
| Keywords | Adenoviridae, Cytoplasm, Dependovirus, Dyneins, Microtubules |
| Abstract | During infection, adenovirus-associated virus (AAV) undergoes microtubule-dependent retrograde transport as part of a program of vectorial transport of viral genome to the nucleus. A microtubule binding assay was used to evaluate the hypothesis that cytoplasmic dynein mediates AAV interaction with microtubules. Binding of AAV serotype 2 (AAV2) was enhanced in a nucleotide-dependent manner by the presence of total cellular microtubule-associated proteins (MAPs) but not cytoplasmic dynein-depleted MAPs. Excess AAV2 capsid protein prevented microtubule binding by AAV serotypes 2, 5, and rh.10, as well as adenovirus serotype 5, indicating that similar binding sites are used by these viruses. |
| DOI | 10.1128/JVI.00481-06 |
| PubMed ID | 16840360 |
| PubMed Central ID | PMC1563724 |