Construction and characterization of hexon-chimeric adenoviruses: specification of adenovirus serotype.
| Publication Type | Academic Article |
| Authors | Gall J, Crystal R, Falck-Pedersen E |
| Journal | J Virol |
| Volume | 72 |
| Issue | 12 |
| Pagination | 10260-4 |
| Date Published | 12/01/1998 |
| ISSN | 0022-538X |
| Keywords | Capsid, Capsid Proteins, Chimera, Mastadenovirus |
| Abstract | This study has used the strategy of gene replacement to characterize the contribution of the adenovirus (Ad) capsid protein hexon to serotype definition. By replacing the Ad type 5 (Ad5) hexon gene with sequences from Ad2, we have changed the type specificity of the chimeric virus. The type-determining epitopes are primarily associated with loop 1 of hexon and, to a much lesser degree, with loop 2. In spite of the serotype distinctiveness of the chimeric hexon viruses, epitope similarity between the vectors resulted in a low level of cross-reactive neutralizing antibody, which in combination with activated cellular and innate arms of the immune system is sufficient to suppress gene transduction following readministration in vivo. |
| DOI | 10.1128/JVI.72.12.10260-10264.1998 |
| PubMed ID | 9811772 |
| PubMed Central ID | PMC110610 |