Procollagen production and procollagen messenger RNA levels and activity in human lung fibroblasts during periods of rapid and stationary growth.
| Publication Type | Academic Article |
| Authors | Tolstoshev P, Berg R, Rennard S, Bradley K, Trapnell B, Crystal R |
| Journal | J Biol Chem |
| Volume | 256 |
| Issue | 6 |
| Pagination | 3135-40 |
| Date Published | 03/25/1981 |
| ISSN | 0021-9258 |
| Keywords | Lung, Procollagen, RNA, Messenger |
| Abstract | The production of procollagen molecules by human diploid fetal lung fibroblasts (HFL-1 cells) remains constant in both rapid and stationary growth phases. However, log phase cells degrade 3-fold more newly synthesized collagen inside the cell prior to secretion than do stationary phase cells. Procollagen mRNA levels, measured by hybridization with a type I procollagen mRNA-specific complementary DNA, are approximately 2-fold higher in confluent cells than in log phase cells. There are no significant differences in the ability of either log phase or confluent HFL-1 cell procollagen mRNA to be translated in an in vitro cell-free translation system. Therefore, the ability of HFL-1 cells to maintain constant collagen production irrespective of the growth status of the cells results from the combined action of a number of regulatory mechanisms, including changes in procollagen mRNA levels, the utilization of procollagen mRNA, and intracellular procollagen degradation. |
| PubMed ID | 7204395 |