Publication Type Academic Article
Authors Perraud F, Yoshimura K, Louis B, Dalemans W, Ali-Hadji D, Schultz H, Claudepierre M, Chartier C, Danel C, Bellocq J
Journal Oncogene
Volume 7
Issue 5
Pagination 993-7
Date Published 05/01/1992
ISSN 0950-9232
Keywords Antigens, Viral, Tumor, Cell Transformation, Neoplastic, Ependyma, Gene Expression Regulation, Viral, Genes, Regulator, Membrane Proteins, Promoter Regions, Genetic, Simian virus 40
Abstract Transgenic mice bearing a human cystic fibrosis transmembrane conductance regulator (CFTR) promoter-SV40 T antigen fusion transgene were generated in order to localize in vivo the potential oncogenesis linked to the tissue-specific activity of the promoter for the CFTR gene. Surprisingly, the only site of tumors resulting from expression of the reporter onc gene was ependymal cells lining the brain ventricles. SV40 T antigen expression in these cells led to a consistent pathology in the first weeks of age: ependymoma and consequent hydrocephaly. Tumor-derived cell lines were established, characterized and shown to originate from SV40 T antigen-induced ependymoma. No pathological alterations were found in other organs, such as lungs and pancreas, in which cystic fibrosis is pathologically manifest in humans. Such transgenic mice and derived cell lines may represent valid models for analysing (1) the role of SV40 T antigen in ependymoma formation and (2) CFTR function in ependymal cells.
PubMed ID 1373882
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