Rapid assessment of adenovirus serum neutralizing antibody titer based on quantitative, morphometric evaluation of capsid binding and intracellular trafficking: population analysis of adenovirus capsid association with cells is predictive of adenovirus in

Publication Type Academic Article
Authors Vincent T, Harvey B, Hogan S, Bailey C, Crystal R, Leopold P
Journal J Virol
Volume 75
Issue 3
Pagination 1516-21
Date Published 02/01/2001
ISSN 0022-538X
Keywords Adenoviridae, Antibodies, Viral, Capsid
Abstract Neutralizing antiviral antibodies are typically detected on the basis of inhibition of viral function, such as propagation of a viral infection or inhibition of viral gene expression. Evidence is presented that anti-adenovirus neutralizing antibodies can be evaluated by analysis of cell-associated capsids or by analysis of intracellular trafficking of the capsids within 1 h after infection. Quantitative analyses of these morphologic parameters represent rapid, broadly applicable, functional assays for the detection of anti-adenovirus neutralizing antibodies.
DOI 10.1128/JVI.75.3.1516-1521.2001
PubMed ID 11152523
PubMed Central ID PMC114056
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