Rapid assessment of adenovirus serum neutralizing antibody titer based on quantitative, morphometric evaluation of capsid binding and intracellular trafficking: population analysis of adenovirus capsid association with cells is predictive of adenovirus in
| Publication Type | Academic Article |
| Authors | Vincent T, Harvey B, Hogan S, Bailey C, Crystal R, Leopold P |
| Journal | J Virol |
| Volume | 75 |
| Issue | 3 |
| Pagination | 1516-21 |
| Date Published | 02/01/2001 |
| ISSN | 0022-538X |
| Keywords | Adenoviridae, Antibodies, Viral, Capsid |
| Abstract | Neutralizing antiviral antibodies are typically detected on the basis of inhibition of viral function, such as propagation of a viral infection or inhibition of viral gene expression. Evidence is presented that anti-adenovirus neutralizing antibodies can be evaluated by analysis of cell-associated capsids or by analysis of intracellular trafficking of the capsids within 1 h after infection. Quantitative analyses of these morphologic parameters represent rapid, broadly applicable, functional assays for the detection of anti-adenovirus neutralizing antibodies. |
| DOI | 10.1128/JVI.75.3.1516-1521.2001 |
| PubMed ID | 11152523 |
| PubMed Central ID | PMC114056 |