Publication Type Academic Article
Authors Tessier L, Jallat S, Sauvageot M, Crystal R, Courtney M
Journal FEBS Lett
Volume 208
Issue 2
Pagination 183-8
Date Published 11/24/1986
ISSN 0014-5793
Keywords Escherichia coli, alpha 1-Antitrypsin
Abstract Analysis of a series of lambda cII::alpha 1-antitrypsin (alpha 1AT) gene fusions of different sizes showed that increased alpha 1AT expression correlated with the stabilisation of a particular computer-predicted RNA secondary structure. Moreover, significant synthesis of unfused alpha 1AT was achieved by reconstruction of this conformation to permit interaction between the upstream region of the ribosome-binding site and the first part of the alpha 1AT coding sequence. This high-level expression was dependent upon certain silent point mutations in the coding sequence, indicating that RNA primary and secondary structure determinants can operate in concert to dictate the efficiency of protein synthesis.
DOI 10.1016/0014-5793(86)81014-6
PubMed ID 2946602
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