Spontaneous expression of the c-sis gene and release of a platelet-derived growth factorlike molecule by human alveolar macrophages.
| Publication Type | Academic Article |
| Authors | Mornex J, Martinet Y, Yamauchi K, Bitterman P, Grotendorst G, Chytil-Weir A, Martin G, Crystal R |
| Journal | J Clin Invest |
| Volume | 78 |
| Issue | 1 |
| Pagination | 61-6 |
| Date Published | 07/01/1986 |
| ISSN | 0021-9738 |
| Keywords | Gene Expression Regulation, Lung Diseases, Platelet-Derived Growth Factor, Proto-Oncogenes, Pulmonary Alveoli |
| Abstract | Alveolar macrophages from normal individuals and patients with interstitial lung diseases spontaneously expressed a 4.2-kilobase mRNA complementary to the c-sis gene, a proto-oncogene coding for one of the chains of platelet-derived growth factor (PDGF). Concomitantly, these cells released a mediator with the properties of PDGF, including: chemotactic factor for smooth muscle cells whose activity was resistant to heat and acid, but sensitive to reduction; mitogenic (competence) activity for fibroblasts; ability to compete with PDGF for its receptor; and precipitated by an anti-PDGF antibody. While blood monocytes did not contain c-sis mRNA transcripts, monocytes matured in vitro expressed c-sis, consistent with the concept that expression of c-sis occurs during the differentiation of monocytes into alveolar macrophages. Together with the known actions of PDGF, these observations suggest that the c-sis proto-oncogene and its PDGF product are part of the armamentarium available to the alveolar macrophages for normal lung defense and participation in lung inflammation. |
| DOI | 10.1172/JCI112574 |
| PubMed ID | 3722386 |
| PubMed Central ID | PMC329531 |